| Name | Time | Date | Meet |
|---|---|---|---|
| Eric Anderson | 44.22 | Nov. 20, 2021 | Augustana Invitational |
| Benjamin Bolinske | 44.39 | Feb. 20, 2016 | 2016 Summit League Championships |
| Benjamin Bolinske | 44.96 | Feb. 21, 2015 | 2015 Summit League Championships |
| Benjamin Bolinske | 45.32 | Feb. 22, 2014 | 2014 Summit League Championships |
| Will Hogue | 45.75 | Feb. 18, 2012 | Summit League Championships |
| John Murman | 46.17 | March 11, 2006 | 2006 NCAA Division II Championships |
| Mark Garrigues | 49.86 | Nov. 20, 2005 | 2005 Minnesota Invitational |
