| Time | Date | Name | Meet |
|---|---|---|---|
| 23.33 | Nov. 19, 2021 | Lindsey Wagner | Brown vs. Yale |
| 24.38 | Feb. 20, 2019 | Isabella Hindley | Ivy League Championships (W) |
| 24.77 | Feb. 15, 2017 | Heidi VanderWel | Ivy League Championships (W) |
| 25.04 | Feb. 3, 2017 | Jacquelyn Du | Yale vs. Harvard, Princeton |
| 25.10 | March 1, 2013 | Cynthia Tsay | Ivy League Womens Championships |
| 25.61 | Feb. 24, 2012 | Cynthia Tsay | Ivy League Womens Championships |
| 25.84 | Feb. 16, 2007 | Moira McCloskey | Ivy League Championships |
| 26.48 | Feb. 2, 2007 | Moira McCloskey | Harvard, Yale vs. Princeton |
| 26.65 | Feb. 24, 2005 | Moira McCloskey | Women's Ivy League Championships |
| 27.17 | Feb. 26, 2004 | Moria McCloskey | Women's Ivy League Championships |
| 27.19 | Jan. 31, 2004 | Moira McCloskey | Women's Harvard-Yale-Princeton |
| 27.30 | Feb. 27, 2003 | Erin Vandenberg | Women's Ivy League Championships |
| 27.61 | Feb. 21, 2002 | Erin Vandenberg | Women's Ivy League Championships |
| 28.18 | Feb. 22, 2001 | Kimberly Richardson | Ivy League Women's Championships |
