| Time | Date | Name | Meet |
|---|---|---|---|
| 44.65 | Feb. 24, 2017 | Shane Ryan | Big Ten Conference Championships (M) |
| 44.78 | March 28, 2014 | Shane Ryan | NCAA Division I Mens Championships |
| 45.13 | Feb. 28, 2014 | Shane Ryan | Big Ten Conference Championships (M) |
| 45.71 | March 1, 2013 | Sean Grier | Big Ten Conference Mens Championships |
| 46.38 | Dec. 1, 2012 | Sean Grier | Georgia Fall Invitational |
| 46.51 | Nov. 14, 2008 | Pat Schirk | Penn State, Florida State @Virginia |
| 46.84 | March 28, 2008 | Pat Schirk | NCAA Division I Mens Championships |
| 48.54 | March 15, 2007 | Pat Schirk | NCAA Division I Mens Championships |
