| Time | Date | Name | Meet |
|---|---|---|---|
| 41.49 | March 30, 2024 | Connor Foote | NCAA Division I Mens Championships |
| 41.68 | March 5, 2021 | Shaine Casas | American Short Course Championships |
| 42.26 | Nov. 23, 2019 | Adam Koster | Art Adamson Invitational |
| 42.38 | Feb. 23, 2019 | Adam Koster | Southeastern Conference Championships |
| 42.74 | Feb. 23, 2013 | John Dalton | Southeastern Conference Championships |
| 42.87 | Jan. 30, 2009 | Balazs Makany | Southern Methodist @Texas A&M |
| 43.38 | March 29, 2008 | Ozzie Gardner | NCAA Division I Mens Championships |
| 45.19 | March 6, 2003 | Andrew Sullivant | American Short Course Championships |
