| Time | Date | Name | Meet |
|---|---|---|---|
| 24.74 | March 14, 2019 | Rita Naude | CSCAA National Invitational Championship |
| 25.85 | Sept. 15, 2017 | Rita Naude | Southern Illinois vs. Lindenwood |
| 26.37 | Feb. 6, 2016 | Kristina Ksarjian | Southern Illinois vs. Evansville |
| 26.62 | Feb. 7, 2015 | Kristina Ksarjian | Southern Illinois vs. Evansville |
| 26.92 | Feb. 2, 2013 | Charlotte Davies | Southern Illinois vs. Evansville |
| 26.99 | Feb. 4, 2012 | Isabela Castro | Southern Illinois vs. Evansville |
