| Time | Date | Name | Meet |
|---|---|---|---|
| 24.34 | Feb. 15, 2023 | Abbie Shaw | CCSA Championships |
| 24.82 | Feb. 19, 2020 | Payton Keiner | Coastal Collegiate Sports Association Championships |
| 24.96 | Feb. 14, 2018 | Payton Keiner | Coastal Collegiate Sports Association Championships |
| 24.97 | Feb. 18, 2015 | Kendall Hough | Coastal Collegiate Swimming Association Championships |
| 25.44 | Feb. 17, 2012 | Bryeanna Ravettine | Coastal Collegiate Swimming Association Championships |
| 26.38 | Feb. 18, 2011 | Bryeanna Ravettine | Coastal Collegiate Swimming Association Championships |
| 28.30 | Nov. 19, 2010 | Bryeanna Ravettine | Radford Invitational |
| 33.45 | Nov. 6, 2010 | Hannah Yacynych | Liberty vs. Gardner-Webb, VMI |
