| Time | Date | Name | Meet |
|---|---|---|---|
| 53.24 | April 16, 2021 | Audrey Steen | Atlantic 10 Championships |
| 54.36 | Feb. 21, 2020 | Audrey Steen | Atlantic 10 Conference Championships |
| 54.64 | Feb. 22, 2019 | Audrey Steen | Atlantic 10 Conference Championships |
| 55.48 | Feb. 17, 2017 | Hallie McCue | Atlantic 10 Conference Championships |
| 55.82 | Feb. 20, 2015 | Abby Watson | Atlantic 10 Conference Championships |
| 56.87 | Nov. 22, 2014 | Abby Watson | CSU Magnus Invitational |
| 56.93 | Feb. 21, 2014 | Morgan Fink | Atlantic 10 Conference Championships |
| 56.97 | Feb. 24, 2012 | Morgan Claycomb | Atlantic 10 Conference Championships |
| 57.21 | Dec. 3, 2011 | Morgan Claycomb | Zippy Invitational |
| 57.22 | Feb. 19, 2010 | Morgan Claycomb | Atlantic 10 Championships |
