| Name | Time | Date | Meet |
|---|---|---|---|
| Emilie Boisrenoult | 53.33 | Feb. 23, 2024 | Ivy League Championships (W) |
| Emilie Boisrenoult | 53.87 | Dec. 3, 2022 | Zippy Invitational |
| Jenna Immormino | 54.02 | Feb. 21, 2014 | 2014 Ivy League Championships (W) |
| Chiara Spinazzola | 54.09 | Feb. 26, 2010 | Ivy League Women's Championships |
