| Time | Date | Name | Meet |
|---|---|---|---|
| 25.64 | Feb. 20, 2019 | Kierston Farley-Sepe | Missouri Valley Conference Championships |
| 25.73 | Feb. 14, 2014 | Sammy Schuckles | Missouri Valley Conference Championships |
| 26.30 | Feb. 15, 2013 | Sammy Schuckles | Missouri Valley Conference Championships |
| 26.53 | Feb. 17, 2012 | Sammy Schuckles | Missouri Valley Conference Championships |
| 26.63 | Dec. 3, 2011 | Sammy Schuckles | Zippy Invitational |
| 27.52 | Feb. 18, 2011 | Molly Gomberg | Missouri Valley Conference Championships |
| 27.75 | Feb. 12, 2010 | Molly Gomberg | Missouri Valley Conference Championships |
| 28.46 | Nov. 6, 2009 | Jeanette Nolte | Illinois vs. Illinois State |
| 28.65 | Oct. 31, 2009 | Jillian Olson | Illinois State vs. Western Illinois (W) |
| 29.06 | Sept. 26, 2009 | Molly Gomberg | Illinois State Red/White Intrasquad Meet |
