| Time | Date | Name | Meet |
|---|---|---|---|
| 47.49 | Feb. 24, 2024 | Davidson Vincent | Midwest Invitational |
| 47.65 | Nov. 17, 2023 | Davidson Vincent | House of Champions Invitational |
| 48.01 | Feb. 13, 2020 | Ruben Leeuwen | Great Lakes Valley Conference Championships |
| 48.38 | Nov. 24, 2019 | Ruben Leeuwen | Phoenix Fall Classic |
| 48.94 | Feb. 8, 2018 | Colin Williams | Great Lakes Valley Conference Championships |
| 49.30 | Feb. 12, 2016 | Mads Knoblauch | Great Lakes Valley Conference Championships |
| 49.53 | Jan. 10, 2015 | Victor Tarin | Lewis vs. Olivet Nazarene |
| 49.64 | Nov. 22, 2014 | Victor Tarin | House of Champions Invitational |
| 49.73 | Nov. 23, 2013 | Victor Tarin | House of Champions Invitational |
| 50.87 | Oct. 18, 2013 | Victor Tarin | Lewis vs. Missouri S & T |
| 51.80 | Oct. 27, 2012 | Alexander Elston | Notre Dame (Ohio) vs. Malone, Lewis, West Virginia Wesleyan |
| 52.45 | Dec. 3, 2005 | Jevgenijs Pesins | CollegeSwimming.com Classic |
