| Time | Date | Name | Meet |
|---|---|---|---|
| 25.74 | Dec. 2, 2022 | Jabari Ramsey | Valparaiso vs. Butler vs. Lewis |
| 26.15 | Nov. 20, 2020 | Jabari Ramsey | Lewis Time Trials Session 1 |
| 26.95 | Oct. 18, 2020 | Jabari Ramsey | Lewis v St. Ambrose |
| 27.14 | Nov. 10, 2018 | Jarred Stigant | Hillsdale vs. Findlay, Lewis |
| 27.39 | Oct. 22, 2015 | Zach Ballon | William Jewell vs Lewis University |
| 28.02 | Dec. 17, 2013 | Zach Ballon | Lewis vs. William Jewell - Day Two |
