| Time | Date | Name | Meet |
|---|---|---|---|
| 47.71 | Feb. 15, 2024 | Ney Chagas Filho | South Atlantic Conference Championships |
| 47.72 | March 10, 2023 | Ney Chagas Filho | NCAA Division II Championship |
| 47.78 | March 9, 2023 | Ney Chagas Filho | NCAA Division II Championship |
| 48.07 | Feb. 9, 2023 | Ney Chagas Filho | South Atlantic Conference Championships |
| 48.42 | Feb. 8, 2019 | Niklas Martin | Bluegrass Mountain / Carolinas Conference Championships |
| 48.53 | March 15, 2018 | Niklas Martin | NCAA Division II Championship |
| 48.66 | Feb. 15, 2018 | Niklas Martin | Bluegrass Mountain Championships |
| 48.76 | March 10, 2017 | Sebastian Holmberg | NCAA Division II Championship Festival |
| 49.11 | Feb. 10, 2017 | Niklas Martin | Bluegrass Mountain Championships |
| 49.22 | March 13, 2014 | Tim Kniffler | NCAA Division II Championships |
| 49.54 | Feb. 15, 2014 | Tim Kniffler | Bluegrass Mountain Conference Championships |
| 49.82 | March 15, 2012 | Tim Kniffler | NCAA Division II Championships |
| 50.40 | March 11, 2011 | Tim Kniffler | NCAA Division II Championships |
| 50.88 | Feb. 18, 2011 | Tim Kniffler | Bluegrass Mountain Conference Championships |
| 51.57 | March 11, 2010 | Lee Sousa | NCAA Division II Championships |
| 51.81 | March 15, 2007 | Brian Parks | NCAA Division II Championships |
