| Time | Date | Name | Meet |
|---|---|---|---|
| 44.74 | Dec. 2, 2023 | Agustin Rasche | Wooster Invitational |
| 44.95 | Dec. 3, 2022 | Ruben Gonzalez De Oliveira | Wooster Invitational |
| 45.51 | Nov. 22, 2015 | Vladislav Khoroletc | Cleveland State Magnus Cup |
| 46.05 | Nov. 23, 2013 | Thalys Caixeta | Western Kentucky Invitational |
| 46.37 | Nov. 20, 2010 | Brett Oliver | Western Kentucky Invitational |
| 46.79 | March 6, 2010 | Matt Limerick | NAIA National Championships |
| 47.19 | Nov. 22, 2009 | Robert Miller | Hilltopper Invitational |
| 49.18 | Oct. 31, 2009 | Matt Limerick | Western Kentucky vs. Cumberland |
| 49.75 | Oct. 17, 2009 | Matt Limerick | Kentucky vs. Arkansas, Cumberland, Southern Illinois |
