| Name | Time | Date | Meet |
|---|---|---|---|
| alizee pelletier | 29.16 | March 15, 2024 | NCAA Division II Championship |
| alizee pelletier | 29.48 | Feb. 17, 2024 | Sunshine State Conference Championships |
| alizee pelletier | 29.68 | Nov. 18, 2023 | Florida Tech Panther Invitational |
| Haley DeGrace | 29.79 | Feb. 23, 2019 | Sunshine State Conference Championships |
| Haley DeGrace | 30.15 | Nov. 17, 2018 | Panther Invitational |
| Kelsey Gouge | 30.19 | Oct. 25, 2013 | Saint Leo vs. Florida Southern |
