| Time | Date | Name | Meet |
|---|---|---|---|
| 44.67 | Feb. 12, 2022 | Caleb McCarty | North Coast Athletic Conference Championships |
| 45.18 | Feb. 13, 2016 | Elliot Johns | North Coast Athletic Conference Championships |
| 45.29 | Feb. 14, 2015 | Elliot Johns | North Coast Athletic Conference Championships |
| 45.51 | Dec. 4, 2014 | Elliot Johns | Calvin Winter Invitational |
| 45.97 | Feb. 15, 2014 | Elliot Johns | North Coast Athletic Conference Championships |
| 46.08 | Dec. 7, 2013 | Elliot Johns | Calvin College Invitational |
| 46.57 | Feb. 20, 2010 | Craig Vetor | Last Chance Meet: Kenyon |
| 46.94 | Dec. 4, 2009 | Craig Vetor | DePauw Invitational |
| 47.07 | Dec. 5, 2008 | Craig Vetor | TYR/CollegeSwimming Classic |
| 47.79 | Nov. 30, 2007 | Craig Vetor | TYR/CollegeSwimming.com Classic |
| 48.02 | Oct. 28, 2006 | Jordan Blackwell | Carthage vs. Wabash, St. Mary' |
