| Time | Date | Name | Meet |
|---|---|---|---|
| 50.37 | Feb. 24, 2024 | Harrison Hong | NESCAC Mens Championships |
| 51.22 | Dec. 2, 2022 | Harrison Hong | Connecticut Cup |
| 51.59 | Feb. 23, 2018 | Ali Pourmaleki | NESCAC Championships Men |
| 52.00 | Feb. 17, 2017 | Spencer Tang | NESCAC Men's Championships |
| 52.42 | Feb. 21, 2015 | Spencer Tang | NESCAC Men's Championships |
| 52.50 | Feb. 20, 2015 | Spencer Tang | NESCAC Men's Championships |
| 54.14 | Jan. 31, 2015 | Spencer Tang | Wesleyan vs. Coast Guard Academy |
| 54.40 | Feb. 21, 2014 | Nathan Courville | NESCAC Men's Championships |
| 54.71 | Feb. 24, 2012 | Nicholas Huston | New England Small College Athletic Conference Men's Championships |
| 55.05 | Feb. 26, 2011 | Nicholas Huston | New England Small College Athletic Conference Mens Championships |
| 55.13 | Jan. 30, 2010 | Michael Bailey | Wesleyan vs. Bowdoin College and Colby College |
| 55.70 | Dec. 5, 2009 | Michael Bailey | Cardinal Invitational |
| 56.63 | Nov. 21, 2009 | Michael Bailey | Wesleyan, Trinity @ Bates |
| 57.43 | Nov. 24, 2008 | Michael Bailey | Wesleyan @Amherst |
| 57.66 | Nov. 22, 2008 | Michael Bailey | Wesleyan, Bates @Trinity (CT) |
