| Time | Date | Name | Meet |
|---|---|---|---|
| 47.95 | Feb. 10, 2023 | Ben Hilfinger | North Coast Athletic Conference Championships |
| 48.00 | March 19, 2015 | Blake Lehmann | NCAA Division III Championships |
| 48.75 | Dec. 7, 2013 | Casey Hooker | Total Performance Invitational |
| 48.76 | March 21, 2013 | Casey Hooker | NCAA Division III Championships |
| 48.77 | Feb. 15, 2013 | Casey Hooker | North Coast Athletic Conference Championships |
| 49.40 | March 21, 2008 | Robert Alexander | NCAA Division III Mens Championship |
| 50.18 | March 16, 2007 | Robert Alexander | NCAA Division III Mens Championships |
| 52.95 | Nov. 18, 2006 | Robert Alexander | Wabash Invitational |
