| Time | Date | Name | Meet |
|---|---|---|---|
| 51.05 | Feb. 21, 2016 | Kelly Ngo | SCIAC Championships |
| 51.25 | March 21, 2015 | Kelly Ngo | NCAA Division III Championships |
| 51.41 | March 22, 2014 | Michele Kee | NCAA Division III Championships |
| 51.77 | Feb. 22, 2011 | Michele Kee | Southern California Intercollegiate Athletic Conference Championships |
| 52.17 | March 21, 2009 | Jennifer Rinker | NCAA Division III Championships |
| 52.20 | March 11, 2006 | Sarah Heine | NCAA Division III Womens Championships |
