| Time | Date | Name | Meet |
|---|---|---|---|
| 53.39 | March 14, 2024 | Emily Mears-Bentley | NCAA Division II Championship |
| 53.83 | Feb. 15, 2024 | Emily Mears-Bentley | Great Midwest-Mountain East Conference Championship |
| 54.14 | March 9, 2023 | Emily Mears-Bentley | NCAA Division II Championship |
| 54.64 | Dec. 2, 2022 | Emily Mears-Bentley | Calvin Winter Invitational |
| 55.73 | Feb. 13, 2020 | Katy Kouvaris | Great Midwest-Mountain East Conference Championship |
| 55.83 | Feb. 16, 2019 | Katy Kouvaris | Great Midwest-Mountain East Conference Championship |
| 55.94 | March 10, 2016 | Jessica Ward | NCAA Division II Championships |
| 56.15 | March 12, 2015 | Jessica Ward | NCAA Division II Championships |
| 56.47 | Feb. 12, 2015 | Jessica Ward | Great Lakes Intercollegiate Athletic Conference |
| 56.54 | Feb. 13, 2014 | Molly Cade | Great Lakes Intercollegiate Athletic Conference |
| 56.65 | Feb. 10, 2012 | Molly Cade | Great Lakes Intercollegiate Athletic Conference Championships |
| 57.94 | Feb. 12, 2010 | TANYA TIKHONOVA | Great Lakes Intercollegiate Athletic Conference |
