| Time | Date | Name | Meet |
|---|---|---|---|
| 59.80 | March 22, 2024 | Jennah Fadely | NCAA Division III Championship |
| 59.94 | March 17, 2023 | Jennah Fadely | NCAA Division III Championship |
| 59.98 | Feb. 10, 2023 | Jennah Fadely | North Coast Athletic Conference Championships |
| 1:00.36 | Jan. 27, 2023 | Jennah Fadely | Kenyon vs. Miami-OH |
| 1:00.75 | March 18, 2022 | Jennah Fadely | NCAA Division III Championship |
| 1:01.35 | Dec. 1, 2017 | Julia Wilson | Total Performance Invitational |
| 1:01.47 | Dec. 2, 2016 | Julia Wilson | Total Performance Invitational |
| 1:01.79 | Dec. 4, 2015 | Julia Wilson | Total Performance Invitational |
| 1:02.43 | March 23, 2012 | Alisa Vereshchagin | NCAA Division III Championships |
| 1:02.76 | March 20, 2009 | Tracy Menzel | NCAA Division III Championships |
| 1:02.94 | Dec. 5, 2008 | Tracy Menzel | Nike Cup (Kenyon) |
| 1:03.57 | March 14, 2008 | Tracy Menzel | NCAA Division III Womens Championship |
| 1:03.69 | March 9, 2007 | Tracy Menzel | NCAA Division III Womens Championships |
| 1:04.39 | March 10, 2006 | Jessica Connors | NCAA Division III Womens Championships |
