| Time | Date | Name | Meet |
|---|---|---|---|
| 25.01 | March 15, 2023 | Olivia Smith | NCAA Division III Championship |
| 25.26 | March 21, 2018 | Crile Hart | NCAA Division III Championships |
| 25.48 | Feb. 16, 2018 | Crile Hart | North Coast Athletic Conference Championships |
| 25.53 | March 15, 2017 | Summer Otazu | NCAA Division III Championships |
| 25.56 | March 18, 2015 | Celia Oberholzer | NCAA Division III Championships |
| 25.72 | Feb. 14, 2014 | Celia Oberholzer | North Coast Athletic Conference Championships |
| 25.79 | Feb. 17, 2012 | Rachel Flinn | North Coast Athletic Conference Championships |
| 26.36 | Dec. 3, 2011 | Rachel Flinn | Kenyon Invitational |
| 26.50 | Feb. 12, 2010 | Kathryn Haller | North Coast Athletic Conference Championships |
| 26.64 | March 18, 2009 | Brittany Hurd | NCAA Division III Championships |
| 26.88 | March 14, 2008 | Brittany Hurd | NCAA Division III Womens Championship |
| 26.97 | March 10, 2006 | Brittany Hurd | NCAA Division III Womens Championships |
