| Time | Date | Name | Meet |
|---|---|---|---|
| 49.51 | Feb. 15, 2020 | Crile Hart | North Coast Athletic Conference Championships |
| 49.90 | March 19, 2016 | Hannah Lobb | NCAA Division III Championships |
| 50.22 | Dec. 5, 2015 | Hannah Lobb | Total Performance Invitational |
| 50.58 | March 21, 2015 | Hannah Lobb | NCAA Division III Championships |
| 50.60 | March 15, 2008 | Tina Ertel | NCAA Division III Womens Championship |
| 50.84 | March 10, 2007 | Tina Ertel | NCAA Division III Womens Championships |
| 52.37 | March 11, 2006 | Elizabeth Carlton | NCAA Division III Womens Championships |
