| Time | Date | Name | Meet |
|---|---|---|---|
| 48.96 | March 17, 2023 | Torri Huske | NCAA Division I Womens Championship |
| 49.17 | March 18, 2022 | Torri Huske | NCAA Division I Womens Championship |
| 49.43 | Feb. 25, 2022 | Torri Huske | Pacific 12 Championships (W) |
| 50.30 | Nov. 19, 2021 | Torri Huske | NC State/GAC Invitational |
| 50.38 | Feb. 24, 2017 | Janet HU | Pacific 12 Championships (W) |
| 50.89 | March 21, 2014 | Felicia Lee | NCAA Division I Womens Championships |
| 51.24 | Feb. 24, 2010 | Elaine Breeden | Pacific 10 Women's Championships |
| 51.34 | March 20, 2009 | Elaine Breeden | NCAA Division I Womens Championships |
| 51.68 | March 21, 2008 | Elaine Breeden | NCAA Division I Womens Championships |
| 51.72 | March 9, 2007 | Elaine Breeden | NCAA Division I Womens Championships |
