| Time | Date | Name | Meet |
|---|---|---|---|
| 44.20 | March 29, 2024 | Will Modglin | NCAA Division I Mens Championships |
| 44.35 | March 24, 2017 | John Shebat | NCAA Division I Mens Championships |
| 44.58 | March 23, 2017 | John Shebat | NCAA Division I Mens Championships |
| 45.03 | March 28, 2014 | Kip Darmody | NCAA Division I Mens Championships |
| 45.17 | Oct. 31, 2008 | Taylor Taylor | Texas, Indiana @Michigan |
| 46.19 | March 15, 2007 | Tom Sacco | NCAA Division I Mens Championships |
