| Time | Date | Name | Meet |
|---|---|---|---|
| 46.42 | March 15, 2024 | Parker Knollman | NCAA Division II Championship |
| 47.06 | March 14, 2024 | Parker Knollman | NCAA Division II Championship |
| 47.08 | Nov. 15, 2023 | Parker Knollman | Gamecock Invitational |
| 47.86 | March 10, 2023 | Parker Knollman | NCAA Division II Championship |
| 47.90 | Feb. 28, 2021 | Angelo Russo | Shark Invitational |
| 48.02 | March 14, 2014 | Karl Burdis | NCAA Division II Championships |
| 48.10 | March 8, 2013 | Karl Burdis | NCAA Division II Championships |
| 48.43 | March 7, 2013 | Karl Burdis | NCAA Division II Championships |
| 48.74 | Feb. 14, 2013 | Karl Burdis | Sunshine State Conference Championship |
| 48.82 | March 16, 2012 | Berk Kahramen | NCAA Division II Championships |
| 48.99 | March 15, 2012 | Berk Kahramen | NCAA Division II Championships |
| 49.61 | Feb. 17, 2012 | Berk Kahramen | Sunshine State Conference Championship |
| 49.74 | Feb. 16, 2012 | Berk Kahramen | Sunshine State Conference Championship |
| 50.45 | Dec. 18, 2011 | Berk Kahramen | Nova Southeastern Invite |
| 50.53 | March 14, 2008 | Kyle Merritt | NCAA Division II Championships |
| 51.97 | March 10, 2006 | Kyle Merritt | NCAA Division II Championships |
| 52.67 | March 9, 2006 | Kyle Merritt | NCAA Division II Championships |
