| Time | Date | Name | Meet |
|---|---|---|---|
| 25.43 | Jan. 12, 2024 | Isabelle Sering | Tampa vs. Keiser |
| 26.47 | Jan. 7, 2022 | Kristen Wagner | Indian River vs. Tampa vs. Nova Southeastern |
| 26.59 | Jan. 18, 2019 | Isabelle Bularca | Keiser at Tampa |
| 26.87 | Dec. 10, 2011 | Lindsay Calimer | Tampa vs. Wright State |
| 27.81 | Nov. 13, 2010 | Caroline Belli | Rollins vs. Tampa |
| 29.29 | Nov. 13, 2009 | Jennifer Jenkins | Tampa vs. Florida A&M |
