| Time | Date | Name | Meet |
|---|---|---|---|
| 29.56 | Jan. 7, 2022 | Elizaveta Bazarova | Indian River vs. Tampa vs. Nova Southeastern |
| 29.84 | Jan. 18, 2019 | Elizaveta Bazarova | Keiser at Tampa |
| 32.01 | Jan. 11, 2014 | Alexandra Hipolito | Rollins vs. Kansas, Tampa |
| 32.82 | Dec. 10, 2011 | Michelle Walcker | Tampa vs. Wright State |
| 33.47 | Nov. 13, 2010 | Heather Glenday | Rollins vs. Tampa |
| 34.86 | Nov. 13, 2009 | Amy O'Brien | Tampa vs. Florida A&M |
