| Name | Time | Date | Meet |
|---|---|---|---|
| Maxim Tsyfarov | 48.57 | Feb. 17, 2024 | Sunshine State Conference Championships |
| Maurice Grabowski | 48.98 | Feb. 16, 2024 | Sunshine State Conference Championships |
| Julian Chan Quee Lin | 50.03 | Nov. 20, 2021 | Panther Invitational |
| Julian Chan Quee Lin | 51.29 | Oct. 29, 2021 | Florida Tech vs. Lynn vs. Saint Leo |
| Lucas Olivos | 52.29 | Feb. 13, 2021 | Florida Tech vs. Lynn |
| Cam Dunne | 53.36 | Feb. 16, 2020 | Sunshine State Conference Championships |
| Cam Dunne | 54.44 | Dec. 6, 2019 | SCAD Invitational |
| Daniel Varon | 56.23 | Oct. 12, 2019 | NSU Shark Invitational |
