| Time | Date | Name | Meet |
|---|---|---|---|
| 50.81 | March 18, 2017 | Tai Hallstein | NCAA Division III Championships |
| 51.28 | Feb. 12, 2017 | Tai Hallstein | Northwest Conference Championships |
| 51.73 | Feb. 15, 2015 | Tai Hallstein | Northwest Conference Championships |
| 53.26 | Feb. 16, 2014 | Tai Hallstein | Northwest Conference Championships |
| 53.34 | Feb. 12, 2012 | Helen Jenne | Northwest Conference Championships |
| 53.62 | Dec. 4, 2011 | Helen Jenne | Husky Invitational |
| 54.12 | Feb. 27, 2011 | Helen Jenne | Northwest Conference Championships |
| 54.22 | Feb. 14, 2010 | Charlotte Graham | Northwest Conference Championships |
| 55.45 | Nov. 21, 2009 | Lauren Flynn | Northwest Invitational |
| 56.41 | Nov. 15, 2008 | Lauren Flynn | Pacific Lutheran @Whitman |
| 57.36 | Nov. 8, 2008 | Lauren Flynn | Whitman & Mills @Pacific University |
| 58.63 | Nov. 7, 2008 | Chelsea Klinger | Whitman @Linfield |
