| Time | Date | Name | Meet |
|---|---|---|---|
| 47.90 | Feb. 17, 2024 | Matt Fritz | W&L Last Chance Meet |
| 47.96 | Feb. 9, 2024 | Matt Fritz | Old Dominion Athletic Conference Championships |
| 48.64 | March 7, 2021 | Danny Lynch | W&L Championship Time Trial |
| 49.07 | March 22, 2012 | Brian Stirling | NCAA Division III Championships |
| 49.26 | Feb. 17, 2012 | Brian Stirling | Bluegrass Mountain Conference Championships |
| 49.78 | Feb. 18, 2011 | Brian Stirling | Bluegrass Mountain Conference Championships |
| 50.36 | Feb. 19, 2010 | Brian Stirling | Bluegrass Mountain Valley Championships |
| 50.54 | Dec. 3, 2009 | Brian Stirling | Virginia Invitational |
| 50.85 | March 19, 2009 | Brian Stirling | NCAA Division III Championships |
