| Name | Time | Date | Meet |
|---|---|---|---|
| Maria Gutzweiler | 26.35 | Feb. 23, 2024 | CCIW Championships |
| Mackinzee Macho | 26.68 | Feb. 21, 2020 | College Conference of Illinois & Wisconsin Championships |
| Gianna Mcguire | 27.19 | Feb. 15, 2019 | College Conference of Illinois & Wisconsin Championships |
| Gianna Mcguire | 27.52 | Dec. 9, 2017 | Carthage vs. Augustana (IL) |
| Carolyn Kelly | 27.98 | Feb. 11, 2005 | 2005 CCIW Championships |
| Janet Hughes | 28.88 | Feb. 14, 2003 | CCIW Swimming Championships |
| Janet Hughes | 28.96 | Feb. 15, 2002 | 2002 CCIW Championships |
