| Time | Date | Name | Meet |
|---|---|---|---|
| 46.59 | March 17, 2023 | Liam McDonnell | NCAA Division III Championship |
| 46.61 | March 16, 2023 | Liam McDonnell | NCAA Division III Championship |
| 46.95 | Feb. 17, 2023 | Liam McDonnell | Ohio Athletic Conference Championships |
| 47.53 | March 18, 2022 | Liam McDonnell | NCAA Division III Championship |
| 47.74 | March 17, 2022 | Liam McDonnell | NCAA Division III Championship |
| 47.83 | Feb. 17, 2022 | Liam McDonnell | Ohio Athletic Conference Championships |
| 47.99 | March 26, 2021 | Liam McDonnell | Ohio Athletic Conference Championships |
| 48.43 | March 25, 2021 | Liam McDonnell | Ohio Athletic Conference Championships |
| 48.65 | Feb. 13, 2020 | Liam McDonnell | Ohio Athletic Conference Championships |
| 49.01 | March 22, 2019 | Forrest Campbell | NCAA Division III Championships |
| 49.51 | Feb. 15, 2019 | Forrest Campbell | Ohio Athletic Conference Championships |
| 49.62 | Feb. 14, 2019 | Forrest Campbell | Ohio Athletic Conference Championships |
| 50.42 | Feb. 17, 2017 | Jack Garrow | Ohio Athletic Conference Championships |
| 50.74 | Feb. 16, 2017 | Jack Garrow | Ohio Athletic Conference Championships |
| 51.11 | Feb. 19, 2016 | Jack Garrow | Ohio Athletic Conference Championships |
| 51.63 | Feb. 18, 2016 | Jack Garrow | Ohio Athletic Conference Championships |
| 53.33 | Dec. 6, 2015 | Jack Garrow | Fredonia Invitational |
| 53.76 | Oct. 24, 2015 | Jack Garrow | John Carroll vs. Case Western |
| 54.30 | Feb. 20, 2015 | Gabe Nock | Ohio Athletic Conference Championships |
| 54.50 | Feb. 19, 2010 | Blake Pinchot | Ohio Athletic Conference Championships |
| 54.52 | Feb. 18, 2010 | Blake Pinchot | Ohio Athletic Conference Championships |
| 57.19 | Nov. 14, 2009 | Blake Pinchot | Veale Classic |
| 57.73 | Nov. 13, 2009 | Blake Pinchot | Veale Classic |
| 58.24 | Nov. 8, 2008 | Blake Pinchot | John Carroll @Mount Union |
| 59.09 | Oct. 25, 2008 | Blake Pinchot | Ohio Northern @John Carroll |
