| Time | Date | Name | Meet |
|---|---|---|---|
| 43.69 | Feb. 19, 2022 | Liam McDonnell | Ohio Athletic Conference Championships |
| 44.83 | Feb. 15, 2020 | Forrest Campbell | Ohio Athletic Conference Championships |
| 45.53 | Feb. 16, 2019 | Matthew Ramsey | Ohio Athletic Conference Championships |
| 45.55 | Feb. 15, 2014 | David Holvey | Ohio Athletic Conference Championships |
| 45.82 | Feb. 19, 2011 | Alex Vereshchagin | Ohio Athletic Conference Championships |
| 46.68 | Feb. 20, 2010 | Alex Vereshchagin | Ohio Athletic Conference Championships |
| 49.11 | Nov. 7, 2009 | Alex Vereshchagin | John Carroll vs. Mount Union |
| 49.23 | Jan. 17, 2009 | Alex Vereshchagin | John Carroll & Allegheny @Oberlin |
| 49.92 | Nov. 8, 2008 | Alex Vereshchagin | John Carroll @Mount Union |
