| Time | Date | Name | Meet |
|---|---|---|---|
| 52.26 | Feb. 18, 2023 | Mairin Dalziel | Ohio Athletic Conference Championships |
| 52.52 | Feb. 15, 2014 | Victoria Watson | Ohio Athletic Conference Championships |
| 53.39 | Feb. 18, 2012 | Karyn Adams | Ohio Athletic Conference Championships |
| 53.78 | Feb. 19, 2011 | Julia Adams | Ohio Athletic Conference Championships |
| 54.32 | Feb. 20, 2010 | Rachel Mizner | Ohio Athletic Conference Championships |
| 56.29 | Jan. 17, 2009 | Andrea Kovacs | John Carroll & Allegheny @Oberlin |
| 57.22 | Oct. 25, 2008 | Andrea Kovacs | Ohio Northern @John Carroll |
