| Time | Date | Name | Meet |
|---|---|---|---|
| 55.73 | March 22, 2019 | Lilly King | NCAA Division I Womens Championships |
| 55.88 | Feb. 22, 2019 | Lilly King | Big Ten Conference Championships (W) |
| 56.25 | March 16, 2018 | Lilly King | NCAA Division I Womens Championships |
| 56.30 | Feb. 17, 2017 | Lilly King | Big Ten Conference Championships (W) |
| 56.85 | March 18, 2016 | Lilly King | NCAA Division I Womens Championships |
| 57.35 | Feb. 19, 2016 | Lilly King | Big Ten Conference Championships (W) |
| 59.39 | March 21, 2014 | Bronwyn Pasloski | NCAA Division I Womens Championships |
| 59.40 | Feb. 20, 2009 | Sarah Stockwell | Big Ten Championships (Women) - Day Three - Finals |
| 1:02.07 | March 21, 2008 | Sarah Stockwell | NCAA Division I Womens Championships |
| 1:02.28 | March 9, 2007 | Sarah Stockwell | NCAA Division I Womens Championships |
