| Time | Date | Name | Meet |
|---|---|---|---|
| 44.75 | March 24, 2023 | Victor Baganha | NCAA Division I Mens Championships |
| 45.18 | March 25, 2022 | Victor Baganha | NCAA Division I Mens Championship |
| 45.19 | Feb. 25, 2022 | Victor Baganha | Big Ten Conference Championships (M) |
| 45.92 | Nov. 19, 2021 | Victor Baganha | NC State/GAC Invitational |
| 46.00 | March 1, 2013 | Sean Grier | Big Ten Conference Mens Championships |
| 46.79 | Dec. 1, 2012 | Sean Grier | Georgia Fall Invitational |
| 46.83 | Feb. 24, 2012 | Sean Grier | Big Ten Conference Mens Championships |
| 47.43 | Feb. 25, 2011 | Sean Grier | Big Ten Conference Mens Championships |
| 47.56 | Feb. 26, 2009 | Tim Williams | Big Ten Championships (Men) - Day Two - Prelims |
| 48.45 | Nov. 14, 2008 | Tim Williams | Penn State, Florida State @Virginia |
| 49.28 | March 6, 2003 | Matthew Haupt | American Short Course Championships |
