| Name | Time | Date | Meet |
|---|---|---|---|
| Lillie Nesty | 24.30 | March 9, 2024 | Florida Spring Senior Championships |
| Sherridon Dressel | 24.34 | March 24, 2021 | 2021 ISCA International Senior Cup |
| Julia Rodriguez | 24.39 | March 2, 2019 | Florida Swimming Senior SCY Championships |
| Cathleen Li | 24.79 | March 16, 2017 | NCSA Spring Junior National Championship |
| Lindsey Trematerra | 25.72 | Feb. 28, 2015 | Florida Senior Championships |
| Renate DuPlessis | 26.15 | Nov. 14, 2003 | FL GSC Gator Fall Classic |
| Zeina Shanata | 26.89 | Dec. 7, 2001 | FL GSC Gator Holiday Classic |
