| Name | Time | Date | Meet |
|---|---|---|---|
| Caeleb Dressel | 42.03 | March 27, 2021 | 2021 ISCA International Senior Cup |
| Brad Deborde | 43.90 | Dec. 6, 2015 | FL GSC Holiday Classic |
| Shaune Fraser | 44.59 | Dec. 4, 2010 | USA Swimming Short Course National Championships |
| Alex Lopez | 45.47 | Nov. 14, 2003 | FL GSC Gator Fall Classic |
| Nathan Summers | 46.06 | Dec. 7, 2001 | FL GSC Gator Holiday Classic |
