| Name | Time | Date | Meet |
|---|---|---|---|
| Josh Fleagle | 43.88 | March 13, 2022 | Florida Swimming Spring Senior Championship |
| Josh Fleagle | 44.93 | Sept. 26, 2021 | FL PSA Planet Swim Season Opener |
| James Peek | 45.09 | Dec. 6, 2014 | AT&T Winter Nationals - College Results |
| Ryan Lochte | 41.99 | Dec. 6, 2008 | ConocoPhillips Short Course Championships |
| Ryan Lochte | 45.23 | Jan. 13, 2007 | Toyota Grand Prix |
| Ryan Lochte | 46.26 | Dec. 7, 2001 | FL GSC Gator Holiday Classic |
| Matt Norton | 54.42 | Sept. 21, 2001 | Daytona Beach Swimming Fall Invite |
