| Name | Time | Date | Meet |
|---|---|---|---|
| Gabby Deloof | 49.35 | Nov. 19, 2022 | Tennessee Invitational |
| Julia Burroughs | 49.55 | March 7, 2021 | NAC Southern SC Championship |
| Julia Burroughs | 50.27 | March 8, 2020 | 2020 GA DYN Speedo Southern Premier |
| Faith Johnson | 49.00 | Dec. 7, 2013 | 2013 AT&T Winter National Championships |
| Ally Hardesty | 51.69 | Dec. 10, 2011 | Speedo Junior National Championships |
| Jenny Darby | 51.78 | Dec. 11, 2010 | Speedo Winter Junior Championships - East |
| Jenny Darby | 52.62 | Feb. 25, 2010 | Southeastern LSC Championships |
| Ally Hardesty | 52.81 | Dec. 12, 2009 | Speedo Junior Nationals |
