| Name | Time | Date | Meet |
|---|---|---|---|
| Noah Nichols | 50.82 | Feb. 17, 2023 | Atlantic Coast Championships |
| Keefer Barnum | 51.09 | March 26, 2021 | NCAA Division I Men's Championships |
| Noah Nichols | 51.36 | Feb. 26, 2021 | Atlantic Coast Conference Men's Championships |
| Noah Nichols | 51.88 | March 26, 2021 | NCAA Division I Men's Championships |
| Yannick Kaeser | 52.47 | Dec. 6, 2014 | Georgia Invitational |
| Yannick Kaeser | 52.88 | Feb. 28, 2014 | 2014 Atlantic Coast Conference Championships (M) |
| Vanja Rogulj | 53.38 | March 15, 2007 | 2007 NCAA Division I Mens Championships |
