| Name | Time | Date | Meet |
|---|---|---|---|
| Matt Brownstead | 41.22 | March 26, 2022 | NCAA Division I Mens Championship |
| Matt Brownstead | 41.87 | Feb. 27, 2021 | Atlantic Coast Conference Men's Championships |
| Matt Brownstead | 42.03 | Nov. 21, 2020 | Tennessee vs. Alabama, Virginia |
| Scot Robison | 42.21 | Feb. 27, 2010 | Atlantic Coast Conference Men's Championships |
