| Name | Time | Date | Meet |
|---|---|---|---|
| Mariana Navarrete | 59.83 | Nov. 1, 2019 | Rollins vs. Saint Leo |
| Nicole Weber | 1:00.58 | Jan. 31, 2015 | Saint Leo vs. Rollins |
| Nicole Weber | 1:01.48 | Jan. 17, 2014 | St. Leo vs. Florida Tech, Lynn |
| Paula Carvajal | 1:02.85 | Jan. 26, 2013 | Saint Leo vs. Rollins |
| Sandra Stopczynska | 1:02.91 | Nov. 5, 2011 | Saint Leo vs. Florida Tech |
