| Name | Time | Date | Meet |
|---|---|---|---|
| Isabelle Sering | 25.34 | March 18, 2021 | NCAA Division II Championships |
| Isabelle Sering | 25.75 | Feb. 28, 2021 | Florida Tech vs. Saint Leo #2 |
| Zofia Pilarska | 25.92 | Feb. 14, 2020 | Sunshine State Conference Championships |
| Karina Kolb | 25.96 | Oct. 26, 2018 | Rollins vs. St. Leo |
| Paula Carvajal | 27.69 | Jan. 17, 2014 | St. Leo vs. Florida Tech, Lynn |
| Ally Black | 28.09 | Oct. 19, 2013 | Nova Southeastern vs. St. Leo |
| Chloe Pellican | 28.36 | Feb. 3, 2012 | Saint Leo vs. Rollins |
