| Time | Date | Name | Meet |
|---|---|---|---|
| 43.41 | March 16, 2024 | Jameson McEnaney | NCAA Division II Championship |
| 43.63 | Dec. 19, 2023 | Ben Sampson | UNLV Invitational |
| 44.07 | Nov. 19, 2022 | Mado Elkady | TYR Colorado Mesa Inviational |
| 44.32 | Feb. 12, 2022 | Mado Elkady | Rocky Mountain Athletic Conference Championships |
| 44.53 | Feb. 20, 2021 | Ben Sampson | Rocky Mountain Athletic Conference Championships |
| 44.72 | March 16, 2019 | Arthur Cury | NCAA Division II Championship |
| 44.75 | Feb. 16, 2019 | Arthur Cury | Rocky Mountain Athletic Conference Championships |
| 44.81 | March 15, 2014 | Kent Olsen-Stavrakas | NCAA Division II Championships |
| 45.07 | Feb. 16, 2013 | Mehdi El Hazzaz | Rocky Mountain Athletic Conference Championships |
| 45.78 | Feb. 18, 2012 | Kent Stavrakas | Rocky Mountain Athletic Conference Championships |
| 46.75 | Oct. 8, 2011 | Kent Stavrakas | Intermountain Shootout |
| 46.80 | Feb. 12, 2011 | Kent Stavrakas | Rocky Mountain Athletic Conference Championships |
| 47.59 | Oct. 23, 2010 | Kent Stavrakas | Rocky Mountain Invitational |
