| Name | Time | Date | Meet |
|---|---|---|---|
| Susana Hernandez Barradas | 49.47 | Feb. 24, 2024 | Missouri Valley Conference Championships |
| Sierra Forbord | 49.97 | April 16, 2021 | Missouri Valley Conference Championships |
| Sierra Forbord | 50.12 | March 19, 2021 | SIU vs UE and Illinois State |
| Therese Mattsson | 50.41 | Feb. 14, 2009 | 2009 Missouri Valley Conference Championships - Day Three - Prelims |
| Therese Mattsson | 50.59 | Dec. 7, 2008 | 2008 Missouri Invite |
