| Time | Date | Name | Meet |
|---|---|---|---|
| 52.14 | Feb. 22, 2019 | Isabella Hindley | Ivy League Championships (W) |
| 52.45 | Feb. 16, 2018 | Heidi VanderWel | Ivy League Championships (W) |
| 52.79 | Feb. 17, 2017 | Heidi VanderWel | Ivy League Championships (W) |
| 54.07 | Nov. 19, 2015 | Isabella Hindley | Nike Cup |
| 54.72 | Feb. 28, 2013 | Cynthia Tsay | Ivy League Womens Championships |
| 54.80 | Feb. 26, 2010 | Emily Dominski | Ivy League Women's Championships |
| 55.62 | Feb. 16, 2007 | Moira McCloskey | Ivy League Championships |
| 55.67 | Feb. 15, 2007 | Moira McCloskey | Ivy League Championships |
| 55.76 | Feb. 23, 2006 | Moira McCloskey | Women's Ivy League Champs |
| 56.23 | Feb. 24, 2005 | Moira McCloskey | Women's Ivy League Championships |
| 57.02 | Feb. 27, 2003 | Erin Vandenberg | Women's Ivy League Championships |
| 57.74 | Feb. 21, 2002 | Erin Vandenberg | Women's Ivy League Championships |
| 58.99 | Feb. 22, 2001 | Kimberly Richardson | Ivy League Women's Championships |
