| Name | Time | Date | Meet |
|---|---|---|---|
| Brandon Dyck | 21.78 | March 10, 2022 | NCAA Division II Championship |
| Brandon Dyck | 21.80 | Feb. 18, 2022 | Sunshine State Conference Championships |
| Brandon Dyck | 22.29 | March 14, 2019 | NCAA Division II Championship |
| Talor Hamilton | 22.41 | Feb. 22, 2019 | Sunshine State Conference Championships |
| Allan Gutierrez | 22.70 | Oct. 25, 2013 | Saint Leo vs. Florida Southern |
