| Time | Date | Name | Meet |
|---|---|---|---|
| 46.43 | Feb. 21, 2020 | Colin Demers | Colonial Athletic Association Championships |
| 47.35 | Feb. 27, 2015 | Will Manion | Colonial Athletic Association Championships |
| 47.82 | March 1, 2013 | Will Manion | Colonial Athletic Association Championships |
| 49.23 | Nov. 16, 2012 | Will Manion | Hokie Fall Invitational |
| 49.64 | Feb. 24, 2012 | Kyle McVeigh | Colonial Athletic Association Championships |
| 49.78 | Feb. 26, 2010 | Jake Maloy | Colonial Athletic Association Championships |
| 50.24 | Feb. 27, 2009 | Shawn Matthews | Colonial Athletic Association Championships - Day Three - Finals |
