| Time | Date | Name | Meet |
|---|---|---|---|
| 49.97 | March 19, 2015 | Blake Lehmann | NCAA Division III Championships |
| 50.39 | Dec. 5, 2014 | Blake Lehmann | Total Performance Invitational |
| 50.81 | Feb. 16, 2012 | Casey Hooker | North Coast Athletic Conference Championships |
| 52.29 | Feb. 3, 2012 | Casey Hooker | De Pauw vs. Rose-Hulman |
| 52.55 | Feb. 18, 2011 | Cam Wiethoff | Southern Collegiate Athletic Conference Championships |
| 52.95 | Feb. 17, 2011 | Cam Wiethoff | Southern Collegiate Athletic Conference Championships |
| 53.06 | Feb. 19, 2010 | Steve Klabak | Southern Collegiate Athletic Conference Championships |
| 53.28 | Feb. 18, 2010 | Steve Klabak | Southern Collegiate Athletic Conference Championships |
| 54.85 | Dec. 4, 2009 | Charlie Boehme | DePauw Invitational |
| 55.85 | Nov. 21, 2009 | Steve Klabak | Wabash Invitational |
| 56.04 | Nov. 11, 2009 | Johnny Montgomery | DePauw vs. Wabash |
| 56.16 | Oct. 31, 2009 | Johnny Montgomery | Washington (Mo) vs. De Pauw, St. Louis |
| 56.23 | Nov. 12, 2008 | Luke Bretscher | De Pauw @Wabash |
